Application of a high-throughput quantitative PCR system for simultaneous monitoring of SARS-CoV-2 variants and other pathogenic viruses in wastewaterArticle Published on 2022-12-202022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] acute respiratory syndrome Alpha variant analyzed application applied assays can be used collected Community coronavirus coronavirus disease COVID-19 delta variant demonstrated detect eight epidemiologically FIVE Health High-throughput qPCR highlighting increase Japan L452R labor-intensive likelihood Mie N501Y nine nucleotide nucleotide substitution pathogenic virus pathogenic viruses Pathogenic viruses, SARS-CoV-2 Patient PCR positive prevalent qPCR quantitative PCR quarantine reported RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant separated Surveillance target tested time-consuming Treatment virus viruses Volume wastewater Wastewater. Yokkaichi [DOI] 10.1016/j.scitotenv.2022.158659 PMC 바로가기
Infectivity of SARS-CoV-2 on Inanimate Surfaces: Don’t Trust Ct ValueArticle Published on 2022-12-192023-07-11 Journal: International Journal of Environmental Research an [Category] COVID19(2023년), [키워드] Ct value Disinfection quantitative PCR SARS-CoV-2 surface contamination. [DOI] 10.3390/ijerph192417074 PMC 바로가기
Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 DiagnosisReview Published on 2022-12-162023-07-11 Journal: Genes [Category] COVID19(2023년), [키워드] COVID-19 Molecular diagnosis polymerase chain reaction quantitative PCR real-time PCR. SARS-CoV-2 [DOI] 10.3390/genes13122387 PMC 바로가기 [Article Type] Review
Effect of oral antiseptics in reducing SARS-CoV-2 infectivity: evidence from a randomized double-blind clinical trialRandomized Controlled Trial Published on 2022-12-012022-10-05 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 임상, [키워드] antiseptic mouthwashe cell cultures cetylpyridinium chloride clinical trial COVID-19 COVID-19 patients Culture double-blind Effect evaluated Evidence in vitro in vivo indicated Infectivity Mouthwash mouthwashe mouthwashes not affected Placebo placebo-controlled clinical trial quantitative PCR Randomized reduce reduced reducing Saliva Saliva. salivary SARS-CoV-2 shown significantly tested viability viral infectivity Viral load Viral transmission virucidal [DOI] 10.1080/22221751.2022.2098059 PMC 바로가기 [Article Type] Randomized Controlled Trial
Randomized, Double-Blind, Placebo-Controlled Trial of a Throat Spray with Selected Lactobacilli in COVID-19 OutpatientsArticle Published on 2022-10-262022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] acting acute respiratory syndrome added Administered administration Amplicon sequencing analyses Analysis antibody Antiviral antiviral property antiviral strategy applied Bacteria benefit Care chills collected Combined coronavirus coronavirus disease COVID-19 COVID-19 pandemic COVID-19 patient COVID-19 patients detectable Diagnosis diarrhea double-blind dried blood spot eligible evaluated Fever Health hospital Hospitalization infected with SARS-CoV-2 Lactobacillaceae lactobacilli Microbiome modulation multifactorial nasopharyngeal viral load nose-throat swab online questionnaire Outpatient Pain Patient per-protocol analysis Placebo placebo-controlled trial positive potential mechanism practitioners qPCR quantitative PCR Randomized reduce reducing reported reported symptom respiratory tract infection respiratory viral disease SARS-CoV-2 selected sequence severity significantly spray strain Symptom symptom resolution the patient the placebo group therapy Throat throat spray. Treatment Trial trials upper respiratory tract Vaccines variant Viral diseases viral infection Viral load was performed [DOI] 10.1128/spectrum.01682-22 PMC 바로가기
Development of a low-cost multi-channel nucleic acid detection PCR instrument and clinical detection application of COVID-19Article Published on 2022-10-092022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, [키워드] amplification COVID-19 COVID-19 outbreak development disadvantage Double channel experiment hospital Low-cost novel Novel coronavirus Nucleic acid detection optical PCR Quantitative quantitative PCR quantitative PCR instrument Real-time fluorescence quantitative PCR. variety were used [DOI] 10.1016/j.aca.2022.340338 PMC 바로가기
IL27 gene expression distinguishes multisystem inflammatory syndrome in children from febrile illness in a South African cohortArticle Published on 2022-09-062022-11-15 Journal: Frontiers in Immunology [Category] SARS, 유전자 메커니즘, 치료제, [키워드] African Anti-inflammatory Antiviral change children clinically Cohort condition conditions Control controls COVID-19 cytokine differential expression differentially expressed gene down-regulated gene expression febrile febrile illness Follow-up Gene Expression healthy healthy children healthy controls highlight IL-27 IL27 immune gene immune-related gene Inflammatory inflammatory condition inflammatory conditions inflammatory reaction innate immune pathway innate immune system investigated Kawasaki disease lack longitudinal MIS-C multisystem inflammatory syndrome. Other pathway Patient pyroptosis quantitative PCR resolved SARS-CoV-2 SARS-COV-2 infection Sensitivity and specificity South South Africa specimen syndrome systemic lupus erythematosus timepoints Treatment two group unique up-regulated was determined [DOI] 10.3389/fimmu.2022.992022 PMC 바로가기
Developing a Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Seven Respiratory Viruses including SARS-CoV-2Article Published on 2022-09-052022-11-15 Journal: Medicina [Category] SARS, 신종인플루엔자, 치료기술, 치료제, [키워드] Accuracy acute respiratory syndrome Adenovirus amplification caused coronavirus coronavirus disease COVID-19 cross-reactivity detection develop Developing diagnosis of COVID-19 evaluate facilitate Final H1N1 H3N2 heat-inactivated include Infection Influenza initial symptom isothermal isothermal amplification material median multiplex Nasopharyngeal swab objective overlap pandemic Patient patients PCR performed point-of-care polymerase chain Quantitative quantitative PCR Rapid reagent respiratory respiratory infection respiratory syncytial virus respiratory virus respiratory virus Respiratory virus. respiratory viruses RSV RT-PCR RT-qLAMP RT-qPCR RT-qPCR assay SARS-CoV-2 Sensitivity and specificity Seven specimen strain Symptom target specificity time-consuming Treatment Volume was performed were used [DOI] 10.3390/medicina58091224 PMC 바로가기
Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus DetectionArticle Published on 2022-09-012022-11-15 Journal: Emerging Infectious Diseases [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료제, [키워드] 95% CI adaptation automated Cobas country detection diagnostic digital PCR Germany Hemisphere high-throughput PCR testing Infrastructure limit of detection molecular molecular diagnostics Monkeypox Monkeypox virus PCR PCR test platform positive quantified quantitative PCR Rapid reported Research rising SARS-CoV-2 serial dilution Spread Streamline swab samples Testing virus viruses zoonoses zoonoses. [DOI] 10.3201/eid2809.220917 PMC 바로가기
Harmonization of SARS-CoV-2 reverse transcription quantitative PCR tests to the first WHO international standard for SARS-CoV-2 RNASARS-CoV-2 RNA에 대한 최초의 WHO 국제 표준에 SARS-CoV-2 역전사 정량 PCR 테스트의 조화Article Published on 2022-09-012022-09-11 Journal: Journal of Clinical Virology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, [키워드] 95% CI 95% confidence interval Analysis assays association calibration Cepheid clinical samples comparable confidence Ct value Ct values cycle cycle threshold Emergency use evaluate Generalizability generate greater harmonization IMPROVE International international standard interquartile range interval IQR log mean difference median nucleocapsid platform positive quantitation Quantitative quantitative PCR Range Regression reverse transcription RT-qPCR RT-qPCRs SARS-CoV-2 SARS-CoV-2 RNA Standard target targets the mean the WHO threshold upper respiratory viral burden was tested WHO [DOI] 10.1016/j.jcv.2022.105242 PMC 바로가기 [Article Type] Article